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PDMS MANUAL PDF

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Pdms Manual Pdf

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New manual at this PDMS version The manual gives an introduction to what PDMS does and .. resourceone.info, which is a contents list for the documentation set. Pdms Manual Pdf Download | PDMS TRAINING AND E3D TUTORIAL. Pipework Design Using. VANTAGE PDMS Version pdmsSP1/man6/doc1 issue. CONN copy command copy nozzle Course Create create pipe pdms 12 design Pdms Manual Pdf Download | PDMS TRAINING AND E3D TUTORIAL.

This window also has its own tool bar. Status Bar This displays information about the current status of your operations. You specify which elements of your design you wish to display, by adding them to or removing them from the draw list.

Pdms Manual

The sample database associated with this exercise represents the whole of a simple building. You should see the Draw List come up in a separate floating window.

If you wish, you can dock this window. Make sure that in the Design Explorer you have expanded any element to display the Graphics below it. This adds selected elements to the Draw List as well as to Graphical View window Alternatively, you can click the right or left mouse-button and drag-and-drop the element into the 3D View.

You can now use the controls in the Draw List to set the color from the popup palette.

Make the floor Black. See the online help for the Design Explorer for details of how to do this. Set the color of the walls to aquamarine.

Pdms Manual

Your building now looks like this: Observe the effect of selecting different view directions from the Look and Isometric menu options provided by the 3D View shortcut menu. You can manipulate the displayed model view in a number of ways. The three view manipulation modes are: Pan the view across the display area Zoom in or out to magnify or reduce the view. Doc No: A4-Rev0 Page 15 of Plant Design Management System Training Guide The current manipulation mode is shown in the status line at the bottom of the 3D View window, and is currently set to Rotate, as shown in the previous illustration.

To change the view manipulation mode, look at the Middle Button Drag options on the 3D View shortcut menu.

By pressing and holding down the middle mouse button with the pointer within the 3D View, the view can manipulated in the selected way simply by moving the mouse. Alternatively, you can change the manipulation mode by pressing one of the function keys, or by using the View Manipulation tool bar buttons, thus: F2 or selects Zoom mode F3 or selects Pan Mode F5 or selects Rotate mode Try these selection options and observe the effect on the Middle Button Drag shortcut menu; a tick appears against the selected option.

Perform the operations while holding down the Ctrl key.

Note that the Word Fast appears in the status line and that the rate of action is increased. Perform the operations while holding down first the Control key to increase the action speed and then the Shift key to decrease the action speed. In the 3D View tool bar, click on the Limits CE button, this adjusts the scale of the view automatically such that it corresponds to a volume the right size to hold the chosen element s ; To set an isometric view direction, position the cursor in the 3D View window and hold down the right-hand mouse button to display the pop-up menu.

Of note, the elongated cell shape of the E. A further increase of the chromate concentration led to biofilms that were dominated by L.

These data indicate that the amount of L. Hence, the results provide a clear demonstration that interdependent consortia self-organize along an autonomously created chemical gradient into spatially separated populations. Figure 4 Interdependent biofilm consortia self-organize along an autonomously created gradient of chromate.

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A series of four identical inocula was used and cultivated for seven days in the absence a,b , or the presence of 1 mM c,d , 2 mM e,f or 3 mM g,h chromate containing medium. The analysis was performed by robotic cell sampling and subsequent staining by the auto FISH procedure. Epifluorescent images and phylogenetic composition in a,c,e,g represent the front section of the microfluidic chip while b,d,f,h show representative data from the rear end. The error bars represent the standard deviation between the ratio of reads from the 16S rDNA amplicon sequencing of two independently cultivated flowcells.

Sampling points are indicated in the scheme of the setup. Unspecific labeling with DAPI is indicated in blue. The bar charts on the left side of each sample represent the community composition derived from 16S rDNA amplicon sequencing. Full size image Productive biofilms Applied biofilm research is concerned with the exploitation of bacterial communities containing novel and superior biocatalysts for biotechnological processes 3.

Hence, to illustrate the scope and utility of our platform, we performed microfluidic cultivation experiments with a productive E. As a test case for the continuous, spatially resolved monitoring of the catalytic activity of the cultured biofilm, we used the sequential biocatalytic reduction of 5-nitrononane-2,8-dione NDK, in Fig. Using the robotic sampler, samples were taken from 12 different points along the meandric cultivation channel.

Together with the results from the metabolic analysis of interdependent biofilms described above, this data clearly illustrates the suitability of our platform for multivariate analysis of complex biofilms. Figure 5 Culturing and analysis of a productive E. The error bars represent the standard deviation of two independent samples, which where withdrawn sequentially from the indicated sampling points.

Full size image Discussion The developed platform for machine-assisted cultivation and analysis of biofilms is ideally suited to advance basic and fundamental research on synthetic as well as natural multispecies biofilms.

Cultivation can be pursued under arbitrary environmental temperature and gas phase conditions to mimic a large variety of natural habitats. Importantly, our system allows for culturing under flow conditions to establish shear forces that are an essential factor for controlling growth in native environments. The standardized dimensions of the flowcell chips allow for the facile integration of a large variety of designs that are readily accessible by rapid prototyping and soft lithography and can be conveniently adjusted in their length, curvature and internal channel dimensions.

This enables diverse applications ranging from rapid growth experiments, as exemplified here with binary E. The here presented biofilm studies show the broad application spectrum of our biofilm analysis platform.

Experiments were routinely run as experimental duplicates or triplicates on individual flow chips. The data obtained clearly indicate that flow culture experiments can be carried out in a reproducible manner so that internal biological processes can be disclosed.

But also with our experimental platform, the results show a variance between the individual cultured biofilms, which indicates that multispecies biofilms are complex life forms that react sensitively to small fluctuations in their environment. One important aspect of ongoing work with our platform is therefore to reveal the exact possibilities and limits of reproducibility. Furthermore, since sequential sampling at identical local coordinates is possible Fig.

A very important feature of the flow chips is their technical connectivity to commercial liquid handling systems and established analytical instrumentation for imaging. We used a combination of these opportunities to develop automated FISH- and CARD-FISH protocols that unburden the user from time consuming experimental steps and even allow for increased throughput by multiplexing chip-based cultivation experiments.

OCT analysis was also implemented in the workflow for online monitoring of biofilm growth and to correlate the fluorescence images to the in vivo situation before fixation and labeling. The OCT controlled automatization increases the validity of the acquired images as the scientist is no longer a source for operational errors.

While automated microscopy can conveniently be implemented for image analysis, it might not even be necessary in certain applications because a plate reader output was shown to reliably help to distinguish between different biofilm-populations. Of note, the migration of FISH-probes into denser parts of the biofilms might be diffusion limited and a depletion of the probes while being pumped from the beginning to the end of the chip might occur.

Changing compositions of the biofilm along the flowpath as well as with varying feed medium could be observed.

Furthermore, it could be shown how the online sampling in combination with HPLC can be used to determine concentration levels of metabolic compounds and biotechnologically-relevant products. On the one hand, this approach gives access to the composition and dynamics of the microbial community cultivated within the flowcells and, on the other hand, it demonstrates the possibility to evaluate online process parameters for biocatalytic transformation reactions.

The latter is considered one of the basic prerequisites for the breakthrough of the use of productive biofilms in biotechnological applications. In summary, microfluidic biofilm cultivation devices and tailored robotic instrumentation were combined with powerful standard methods such as CARD- FISH, HPLC and next generation sequencing to create a unique set of tools for multispecies biofilm research.

PDMS-Training.pdf

All here described techniques for biofilms analysis do not rely on the genetic labelling of cells and, therefore, enable the investigation of native communities. Emerging research fields, for example, studies on productive biofilms or the exploration of complex native consortia will largely benefit from the developed platform.

It is therefore anticipated that this work represents an important step towards the realization of machine-assisted processes for the next generation of biotechnology.

The straight channel for biofilm cultivation was 3 mm wide, 1 mm high and 54 mm long Fig. The layout of the flowcell was designed in accordance with the spacing of a microwell plate, enabling optical analysis with standard microplate readers. The minimal spotsize for fluorescence measurements in the here used microplate reader Infinite M pro, Tecan, Switzerland was 3 mm corresponding to the channel width of the straight cultivation flowcell.

Cannulas Sterican, B. Braun Melsungen AG, Germany , were inserted through horizontal holes in the molds before pouring the PDMS prepolymer to serve as placeholders for the later connection channel.

A dedicated motor controller PS 90, owis, Germany equipped with three control modules for the individual axes was used for operating the movements.

The linear stages were mounted upside down on an optical portal in order to attain a robotic liquid handling arm. Thereby, a customizable layout with fixed positions of all components was realized Supplementary Fig. The exact positions of the fluidic chips were determined automatically by a camera. Two reference points on the chip that were directly incorporated in the replication master were recognized by the image analysis tool AForge.

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This allowed for the calculation of the coordinates of predefined sampling points Supplementary Fig. The calibration of the camera to needle offset was done manually. The robotic sampler system was controlled by a custom-made software with a graphical user interface Supplementary Fig. Further technical details of the robotic setup and the control software will be published elsewhere. In one experiment triplicates of each biofilm where cultivated in parallel. Before inoculation the whole system was equilibrated in medium for at least 12 h.

For the pure culture biofilms, the system was then inoculated with overnight cultures of both species, which were diluted to an optical density of 0.If this rule has not been set up properly, the geometry at that point of connection may not be proper, which is shown in Figure 1. SHEE Each sheet can have several views.

It will consist of the following components, listed in head-to-tail order: Documents that can provide you with further information are listed below.

Note that implied ducting is not. To avoid having to specify this data again for each component, you can set a Default Specification at Pipe or Branch level. First select this menu, then select the Owner option, this will navigate to the owner of the CE.